The Role of Edible Bulbous Layers on Macro, Micro, and Heavy Metal Contents of Leek (Allium porrum) Plant.

In this study, the degree of accumulation of biogenic element and heavy metal contents of different parts and edible layers of leeks cultivated in Konya in Turkey was revealed. The amounts of P and K of leek were determined from 154.69 (leaf top of leek) and 985.05 mg/kg (root of leek) to 1377.63 (onion part of leek) and 2688.50 mg/kg (root of leek), respectively. P and K contents of leek layers changed from 139.45 (1st layer) and 446.63 mg/kg (7th layer) to 1596.69 (2nd layer) and 2201.53 mg/kg (4th layer), respectively. While Ca amounts of leek parts vary between 577.09 (leaf of leek) and 666.87 mg/kg (root of leek), Mg contents of leek parts were determined between 130.70 (onion part of leek) and 264.58 mg/kg (root of leek). All of the macro elements were detected in the highest amount in the root of the leek, followed by the leaf and bulb parts in decreasing order. Fe and Zn contents of different parts of leeks varied from 0.506 (onion part of leek) and 22.71 mg/kg (root of leek) to 1.53 (leaf top of leek) and 5.85 mg/kg (root of leek), respectively. In general, the heavy metals found in the highest amount both in different parts of the leek and in the edible bulbous layers were As and Ba. The layers of the leeks are rich in potassium, phosphorus, iron, and zinc.

Investigation of accumulation of element contents in some wild and cultivated dried fruits.

In this study, the moisture and biogenic element quantities of most wild and cultivated edible dried fruits were investigated. Macroelements found in highest amounts in fruits were K, P, Ca, and Mg. While K quantities of the fruits are recorded between 5212.77 ("white myrtle") and 25550.60 mg/kg ("black nightshade"), P amounts of the fruits were characterized to be between 949.08 (black myrtle) and 4420.75 mg/kg ("black nightshade"). Ca and Mg amounts of the fruits were assessed to be between 359.83 (plum) and 4330.89 mg/kg ("yellow hawthorn") to 214.98 (plum) and 1852.04 mg/kg ("black nightshade"), respectively. Fe and B quantities of the fruitss were established to be between 2.69 ("black myrtle") and 60.13 mg/kg (cherry) to 3.76 ("black myrtle") and 76.25 mg/kg (sour cherry), respectively. In general, except for white and black myrtle fruits, it is thought that other fruits can be good sources of P, K, and Fe. P contents of "laurel cherry," "cherry," "black nightshade," and "Fragrant black grapes" were found partly high than those of other fruits. "Cherry laurel," "sour cherry," "cherry," "pear," "black nightshade," "black fig," and "hawthorn (yellow)" fruits contain more K than other fruits.

In Vitro Evaluation of Bioavailability of Cr from Daily Food Rations and Dietary Supplements from the Polish Market.

Only some of the nutrients consumed with food are able to be absorbed from the gastrointestinal (GI) tract and enter the systemic circulation (blood). Because some elements are essential minerals for humans, their beneficial effect on the body depends significantly on their bioavailable amount (the fraction that can be absorbed and used by the organism). The term bioavailability, which is very often used to describe the part of nutrients that is able to be absorbed, is influenced by various factors of exogenous and endogenous origin. The main purpose of the study was to assess the relative bioavailability of Cr from selected dietary supplements in the presence of various types of diets, which significantly influence the level of bioavailability. The research was performed using a previously developed and optimized two-stage in vitro digestion model using cellulose dialysis tubes of food rations with the addition of pharmaceutical products. Cr was determined using the ICP-OES and GF-AAS methods, depending on its concentration in particular fractions. The determined relative bioavailability ranged between 2.97 and 3.70%. The results of the study revealed that the type of diet, the chemical form of the molecule, and the pharmaceutical form of preparations have a significant influence on the bioavailability of Cr.

Comparative analysis of heavy metal content of unregulated facial whitening creams and branded facial whitening creams.

The present study was conducted to evaluate potential health risks of Omani cosmetics considering the dangers and adverse effects on health caused by unregulated and unlabelled cosmetic products. Therefore, this current study is to examine the toxic heavy metals of unregulated facial whitening creams that are available in the Omani market and compare them with branded facial whitening creams. A total twenty-two samples were purchased from the Souqs/markets of all eleven governorates of Oman and their heavy metals content was determined by using Inductively Coupled Plasma-Optical Emission Spectroscopy (ICP-OES). The sample was digested by using ultra wave microwave at 180 °C. The percentage of heavy metals present in the unregulated whitening creams were compared with the heavy metals content of two randomly selected and branded whitening creams. The results showed that most of the unregulated samples contain significant amount of chromium, cooper and lead. In comparison, the branded face creams contained only the permissible amount of the heavy metals tested. The maximum arsenic content was found in a sample collected from Al Batinah South region (1.74 ppm). The maximum cadmium content was found in a sample collected from Al Wusta region (2.02 ppm). The maximum cobalt and chromium content were found in the samples collected from Al Buraimi region (0.10 ppm) and Dhofar region (8.04 ppm) respectively. In conclusion, unregulated face creams are not safe for human consumption because they contain significant levels of heavy metals that exceed the permissible limits.

Assessment of essential and potentially toxic metals in raw cow milk from Mukaturi town, Oromia Regional State, Ethiopia.

Cow milk is a complete and highly nutritious source of food for humans. However, the quality of milk products has become a significant health concern for consumers, particularly infants and children, in many developing nations, including Ethiopia. The objective of this study was to determine the heavy metal levels in raw cow milk collected from dairy producers and collection centers in Mukaturi town, Ethiopia. Sixty raw cow milk samples (40.0 milk samples from dairy farms and 20.0 milk samples from collection centers) were randomly collected and digested using a mixture of nitric acid (HNO3), hydrogen peroxide (H2O2) and perchloric acid (HClO4) on a hot plate. The amounts of heavy metals (Cd, Co, Cr, Cu, Fe, Mn, Ni, Pb, and Zn) in the milk samples were determined using inductively coupled plasma atomic emission spectroscopy (ICP-OES). The findings showed that the average concentrations of Pb (2.31 ± 0.127), Fe (0.566 ± 0.130), Ni (0.210 ± 0.0189), Cd (0.0372 ± 0.0230), Cr (0.369 ± 0.0162), and Co (0.225 ± 0.0150) in mgL-1 were higher than the allowable limits. This could pose a health risk to the public. However, the concentrations of Mn (0.044 ± 0.0369), Cu (0.195 ± 0.0450) and Zn (2.90 ± 0.0570) in mg L-1 were lower than or within the recommended limits and cannot pose any threat to consumers. The validity of the digestion processes was checked by the recovery test. The percentage recoveries of Cd, Co, Cr, Cu, Fe, Mn, Ni, Pb, and Zn were found to be in the range of 80.7-122 %, which is within the acceptable range. Therefore, special attention should be given to the continuous monitoring of heavy metal levels in raw milk among dairy producers and collection centers to minimize economic losses and the risk to consumer safety.

Leaves, Infusion, and Grounds-A Three-Stage Assessment of Element Content in Yerba Mate (Ilex paraguariensis) Based on the Dynamic Extraction and Mineralization of Residues.

The more yerba mate infusions that are consumed, the larger the amount of grounds generated. What is more, both the infusion and the residues after brewing remain rich elements. Therefore, a strategy for the three-stage assessment of the element content was presented. A new brewing method was based on dynamic extraction, ensuring both the ease of preparing the infusion and recovering the grounds. In turn, microwave-assisted acid mineralization was used to decompose the leaves and twigs of yerba mate before and after brewing. In total, 30 products were analyzed by ICP OES in three fractions each, i.e., dry yerba mate, infusion, and grounds, to determine up to 25 elements. The elemental composition was considered in terms of the country of origin, type, or composition of yerba mate. The extraction percentages obtained with dynamic extraction were comparable to previously used ultrasound-assisted extraction, as well as data from the literature. The three-stage strategy is a novel approach in yerba mate studies, and it may be a model procedure for the laboratory preparation of yerba mate grounds (waste that can be re-used, e.g., a natural fertilizer).

Elemental Fingerprinting of Pecorino Romano and Pecorino Sardo PDO: Characterization, Authentication and Nutritional Value.

Sardinia, located in Italy, is a significant producer of Protected Designation of Origin (PDO) sheep cheeses. In response to the growing demand for high-quality, safe, and traceable food products, the elemental fingerprints of Pecorino Romano PDO and Pecorino Sardo PDO were determined on 200 samples of cheese using validated, inductively coupled plasma methods. The aim of this study was to collect data for food authentication studies, evaluate nutritional and safety aspects, and verify the influence of cheesemaking technology and seasonality on elemental fingerprints. According to European regulations, one 100 g serving of both cheeses provides over 30% of the recommended dietary allowance for calcium, sodium, zinc, selenium, and phosphorus, and over 15% of the recommended dietary intake for copper and magnesium. Toxic elements, such as Cd, As, Hg, and Pb, were frequently not quantified or measured at concentrations of toxicological interest. Linear discriminant analysis was used to discriminate between the two types of pecorino cheese with an accuracy of over 95%. The cheese-making process affects the elemental fingerprint, which can be used for authentication purposes. Seasonal variations in several elements have been observed and discussed.

Pervasiveness of inorganic gunshot residue (IGSR) in handguns after cleaning and conditioning procedures.

Memory effect in firearms that is, the possibility for a weapon to release inorganic particles whose elemental composition depends on its entire shooting history, is responsible for most of the interpretation difficulties encountered in forensic gunshot residue analysis. The presence of residues chemically inconsistent with the last discharged round, the creation of particles having unusual elemental profiles, and the dependence of residue population composition on the collection point are all manifestations of memory effect. The experimental results reported in this paper highlight the ineffectiveness of a wide number of gun cleaning procedures in reducing memory effect. Moreover, the common alternative of discharging batches of rounds having a "new" primer mixture does not fully eliminate the possibility to recover "old" residues at least from the shooter's hands. Two brand new pistols and ammunition having lead-based, leadless and heavy metal free primers were used. Specimens, collected both from the shooters' hands and from cotton targets set nearby the gun muzzle, were analyzed by SEM-EDS and by ICP-OES. After discharging 10's of new ammunitions, the number of old residues ejected from the gun muzzle indeed showed an asymptotic decrease to zero. In spite of this, the number of old residues recovered from the shooter's hands did not follow any predictable trend. These different behaviors suggest that all internal components of a gun, and not just the barrel, play a role in memory effect.

Comparative analysis of metal pollution in the Atlantic Ocean and Mediterranean Sea: insights from Anemonia sulcata study.

In this study, we investigated metal concentrations in Anemonia sulcata specimens from various locations in the Atlantic Ocean and the Mediterranean Sea. A total of 84 individuals were sampled from specific zones, and their tissue samples were processed for metal analysis using Inductively Coupled Plasma Optical Emission Spectrometry (ICP-OES). The results revealed notable differences in metal concentrations among the studied regions. The Atlantic Ocean and the Mediterranean Sea were found to have distinct patterns of marine pollution, influenced by a complex interplay of geographical, demographic, industrial, and environmental policy factors. Conversely, the semi-enclosed Mediterranean Sea has a lower natural dilution capacity, leading to the accumulation and prolonged presence of pollutants. Population density and industrial activities in coastal areas play a significant role in pollution disparities between the Mediterranean Sea and the Atlantic Ocean. The Mediterranean coasts, with higher population densities and intensive industrial operations, experience greater strain on marine ecosystems due to increased pollution sources. Additionally, environmental policies and management approaches differ between the two regions, contributing to variations in pollution response and regulation.

Investigation of elemental composition in red teff grains using inductively coupled plasma optical emission spectroscopy (ICP OES), Sire District, Arsi zone, Ethiopia.

BACKGROUND: Minerals are important not only for better plant growth and development but also for human and animal nutrition. It is known that east and west Gojam in the Amhara region and east and west Shoa areas in the Oromia region Ethiopia's most teff growing areas. However, there is no information on the mineral content and nutritional worth of Teff Sire district, Arsi zone, Ethiopia. Since ICP OES is a powerful technique to examine elemental compositions even in lower concentration, it is used in this work to investigate the elemental composition of red teff samples. METHODS: The elemental compositions of red Teff grain samples were determined using ICP-OES from three sites: S1, S2, and S3 of Sire district, Arsi zone, Ethiopia. Wet digestion of the teff samples was carried out by weighing 0.5 g red teff sample and digested with 8 ml HNO3 and 2 ml H2O2 (30%) for 3:00 h at a temperature of 100℃ on hot plate. The investigations of method validation, limit of detection and limit of quantification were also carried out. RESULTS: The average amount of elements in red teff sample obtained as 172-280 mg/kg Fe, 13-76 mg/kg Mn, 8.2-8.5 mg/kg Cu, 24-26 mg/kg Zn, and toxic trace elements 0.12-0.29 mg/kg Pb and 0.15-0.22 mg/kg Cd. The limit of detection found in ranges from 0.21 mg Kg-1 to 10.44 mg Kg-1 whereas quantification limit resulted in 0.7 mg Kg-1 to 34.8 mg Kg-1 for the metals under consideration. The method was validated by its linear range in the concentration range of 0.028-1.4 ppm or 0.056-2.8 ppm and excellent recovery result was achieved in the range of 90-120%. CONCLUSION: This study aimed to investigate the mineral content in red teff cultivated in Ethiopia specifically Arsi zone by using ICP OES. From the obtained results, Iron was the first abundant essential element in red teff compared to Mn, Cu and Zn. The level of trace elements: Cd and Pb in the samples slightly above the acceptable limit, possibly due to agricultural practices like usage of fertilizers, pesticides, and other industrial products. Overall, this red teff elemental composition information contributes to the nutrition database and food safety in Ethiopia and beyond.

Concentration of Heavy Metals in Traditional and Industrial Fruit Juices from Iran: Probabilistic Risk Assessment Study.

Exposure to heavy metals can endanger the health of exposed people in the long term. The consumption of fruit juice is increasing; it is important to estimate the health risk of consumers due to heavy metals. The current study was carried out for the analysis of toxic metals (lead (Pb), arsenic (As), and cadmium (Cd)) and essential elements (copper (Cu) and zinc (Zn)) in 60 samples of traditional and industrial fruit juices (10 samples of different brands of apple, orange, grape, peach, mango, and pineapple) in Hamadan, West Iran, using inductivity coupled plasma optical emission spectrometry (ICP-OES) method. The validation protocol included precision of the analytical method; recovery, the determination of the limit of detection (LOD), the limit of quantification (LOQ), and linearity were measured. Moreover, risk assessment was detected using target hazard quotient (THQ) and cancer risk (CR) by the Monte Carlo simulation (MCS) model. The ranking of metal concentration in traditional and industrial fruit juices was Zn > Cu > As > Pb > Cd. In all samples, concentrations of heavy metals in industrial fruit juices were higher than traditional fruit juices p < < 0.001. The level of metals in all samples was lower of the US Environmental Protection Agency (USEPA), the World Health Organization (WHO), and the Iran Standard (IS) permissible limit set for drinking water. In terms of non-carcinogenic, values of toxic elements for children and adult in traditional and industrial fruit juices were 1.6E-3 and 1.72E-3 and 2.6E-3 and 1.85E-3, respectively. The 95th percentile of CR in adults and children due to both industrial and traditional fruits juices was higher than 1E-6; hence, reducing the concentration of As in fruit juices should be conducted. Consumption of fruit juice can increase carcinogenic risk of consumers. Therefore, it is recommended to consume it with caution.

Electrocoagulation using Ti/Ti for the remediation and reuse of aqueous Dispersive Blue-79.

The entire ecology is contaminated by the synthetic dyes that are widely utilised in the textile industries. They can be handled using a variety of technologies, but an eco-friendly method called electrocoagulation has been used to prevent additional contamination. Textile wastewater containing disperse dyes are successfully treated in Electrocoagulation (EC) utilizing Al, Fe, and Stainless Steel (SS), but it is not cost effective, also the treated water contains certain mg/L of the metals used, along with dye components, which obstructs the reuse of the same. The effects of initial pH, applied voltage, dye concentration, supporting electrolyte, and treatment time on the colour removal efficiency (CRE) and consumption of energy were examined in EC process followed by activated charcoal filtration (hybrid process) with a monopolar Ti/Ti electrode on the remediation of aqueous solution of Dispersive Blue-79 (dye 3G). The maximum CREobtained was 99.4%, chemical oxygen demand (COD) 93%, and biological oxygen demand (BOD) 85%, under the following optimized operating conditions, applied voltage 15 V, pH = 7, concentration of dye, electrolyte 110 mg/L, 0.2 g/L and time = 15 min. The overall operating cost for the treatment of aqueous dye 3G was 0.455US/m3. The mechanism of EC was studied using XPS analysis in the sludge obtained. For the purpose of the reuse, FTIR, AAS, and ICP-OES analysis were done and compared with the aqueous dye 3G, after EC and hybrid process to ensure the maximum removal of the degraded dye components and metal. ICP-OES results showed that there were no traces of metal in the treated aqueous dye 3G using this method. Throughout the study, the experimental outcomes indicated that the hybrid process upgraded the quality of the treated aqueous dye 3G.

Analysis and Risk Assessment of Essential and Toxic Elements in Algerian Canned Tuna Fish.

In Algeria, the data and research on the essential and toxic chemical elements in food are limited and insufficient; therefore, the present study focused on evaluating the essential and toxic elements content in different 11 brands with two types (tomato and oil) of canned tuna fish, consumed in Algeria in 2022, by inductively coupled plasma-optical emission spectrometer (ICP-OES), while mercury (Hg) levels were determined by cold vapor atomic absorption spectrophotometry, as well as estimate the probabilistic risk assessment. The elements in canned tuna fish commercialized for human consumption in Algeria were determined by ICP-OES methods, The results showed that the heavy metal concentration in the samples under study ranged from 49.11 to 289.80 mg.kg-1 (Ca), 0.0045 to 0.2598 mg.kg-1 (Cd), 0.128 to 1.21 mg.kg-1 (Cr), 8.55 to 35.94 mg.kg-1 (Fe), 121.27 to 379.17 mg.kg-1 (Mg), 0.0767 to 1.2928 mg.kg-1 (Mn), 2.10 to 3.95 mg.kg-1 (Mo), and 2.86 to 35.90 mg.kg-1 (Zn), whereas Cu, Pb, Cu, Ni, and As were under limit of detection (LOD), while the toxic elements of mercury (Hg) levels were determined by cold vapor atomic absorption spectrophotometry which showed a content ranged from 0.0186 to 0.0996 mg.kg-1. The levels of mineral elements concentration were close to the minimum recommended by Food and Agriculture Organization (FAO). Obtained data for this investigation can be suitable for Algerian food purposes.

Deep eutectic solvent-based ultrasound-assisted extraction in soil samples preparation and elemental determination by ICP-OES.

A fast, simple and green method was established for the extraction of Al, Be, Ca, Co, Cr, Cu, Mn, P, Pb, V and Zn from soil samples using ultrasound-assisted deep eutectic solvents (DESs). DESs based on choline chloride, carboxylic acids and polyols were investigated. It was established that the solvent synthesized from choline chloride and oxalic acid provided the highest extraction recovery (85-104%). Inductively coupled plasma-optical emission spectrometry (ICP-OES) was employed to determine the target analytes in the extracts. The parameters that affect the extraction of target analytes was optimized using standard reference samples. Target analytes could be effectively isolated from soil samples using 0.5 g of DES, ultrasound for 40 min, and heating at 80 â„ƒ. The content of the target analytes determined by this method showed no significant difference from the certified values of 24 reference samples. The proposed method was applied to quantify target analytes in real soil samples. Compared to the traditional acid digestion method, this method showed no significant difference in precision and accuracy, with a confidence level of 95%. The proposed method was found to be simple, accurate and environmentally friendly.

Aqueous two-phase system based on benzethonium chloride and sodium dihexyl sulfosuccinate for extraction and ICP-OES determination of heavy metals.

An aqueous two-phase system (ATPS) based on benzethonium chloride (BztCl) and sodium dihexyl sulfosuccinate (NaDHSS) was proposed for the first time for liquid-liquid microextraction of Cd(II), Co(II), Cu(II), Mn(II), Ni(II), and Pb(II) followed by ICP-OES determination. The mixture of cationic and anionic surfactants, BztCl and NaDHSS, showed liquid-liquid phase separation at the molar ratio of 1:1, and the total surfactant concentration of 0.01-0.2 mol L-1 forming ATPS that was investigated in the extraction process. The extraction efficiency for Cd(II), Co(II), Mn(II), Ni(II), and Pb(II) was nearly 100 %, and for Cu(II) - not lower than 88 % in the presence of 8-hydroxyquinoline as a complexing agent. The surfactant-rich phase containing analytes was subjected to back-extraction with 0.2 M HNO3 before ICP-OES measurements. The preconcentration in the proposed BztCl-NaDHSS-H2O ATPS for 30 s and the high degree of back-extraction, which was achieved in 1 min, significantly reduced the sample preparation time, matrix effects and provided low LODs in the range of 0.04-1.0 µg L-1, the preconcentration factor was 120. The analysis of a certified reference material sample of surface water and the real samples of tap, sea, and waste water verified the method accuracy.

The relationship between lymphocyte DNA damage, coronary artery disease, and blood trace elements.

Somatic DNA damage and causative factors (occupational exposures, foods, habits, etc.) are thought to contribute to the pathogenesis of atherosclerosis, although knowledge about their role in coronary artery disease (CAD) is still insufficient. This study aimed to determine the effects of lymphocyte-DNA damage and blood trace element concentrations on CAD. The single-cell alkaline comet was used in the measuring of the lymphocyte DNA damage in blood samples obtained from patients (n = 99) whose CAD grade was determined by the syntax score while the angiographic intervention was carried out. Blood trace element (n = 14) concentrations were monitored by the inductively coupled plasma-optical emission spectroscopy (ICP-OES) after microwave digestion. The relationship between the DNA damage frequencies of the participants and their syntax scores, blood trace element concentrations, and other demographic and clinic parameters were statistically analyzed. Significant correlations were detected between comet data and syntax score (r = 0.858, P < .001), age (r = 0.337, P < .001), blood-urea (r = 0.360, P < .001), creatinine (r = 0.388, P < .001), HbA1c (0.218, P < .05), ECG-QRS time (r = 0.286, P < .01), ECHO-EF (r = -0.377, P < .001), and platelet (r = -0.222, P < .05). The DNA damage frequencies of the groups formed according to their CAD scores were significantly different from the control group (P < .001) and also each other (P ≤ .01). Comet frequencies and CAD grades were found to be correlated with aging (P < .05). DNA damage frequency and syntax score values were significantly (P < .05) higher in males compared to females. Syntax scores were correlated with aging (r = 0.348, P < .01), ECHO-EF (r = 0.374, P < .001), blood-urea (r = 0.398, P < .001), creatinine (r = 0.433, P < .001), glucose (0.218, P < .05), and HbA1c (r = 0.200, P < .05). Significant correlations were observed between trace elements and demographic values, blood parameters, diseases, angio parameters, ECHO, and ECG parameters. It was observed that the concentrations of trace elements detected in the blood were 93.4% correlated with each other. Lymphocyte DNA damage is a strong biomarker for the atherosclerotic indicator of CAD. Aging is an effective factor both in the DNA damage frequency and CAD risk index. Creatinine and urea are factors that have the power to change the CAD risk index and DNA damage frequency. The higher DNA damage and CAD risk were monitored in males compared to females. The relationship between some biomarkers and blood trace element concentrations showed that further studies are needed to more accurately evaluate the relationship between trace elements, DNA damage frequencies, and CAD.

Evidence of direct interaction between cisplatin and the caspase-cleaved prostate apoptosis response-4 tumor suppressor.

Prostate apoptosis response-4 (Par-4) tumor suppressor protein has gained attention as a potential therapeutic target owing to its unique ability to selectively induce apoptosis in cancer cells, sensitize them to chemotherapy and radiotherapy, and mitigate drug resistance. It has recently been reported that Par-4 interacts synergistically with cisplatin, a widely used anticancer drug. However, the mechanistic details underlying this relationship remain elusive. In this investigation, we employed an array of biophysical techniques, including circular dichroism spectroscopy, dynamic light scattering, and UV-vis absorption spectroscopy, to characterize the interaction between the active caspase-cleaved Par-4 (cl-Par-4) fragment and cisplatin. Additionally, elemental analysis was conducted to quantitatively assess the binding of cisplatin to the protein, utilizing inductively coupled plasma-optical emission spectroscopy and atomic absorption spectroscopy. Our findings provide evidence of direct interaction between cl-Par-4 and cisplatin, and reveal a binding stoichiometry of 1:1. This result provides insights that could be useful in enhancing the efficacy of cisplatin-based and tumor suppressor-based cancer therapies.

Microwave-assisted UV digestion of starch and skimmed milk powder: Environmentally friendly protocol for essential and toxic elements determination.

The present work evaluated a microwave-assisted wet digestion method using diluted HNO3 with in situ UV radiation for the digestion of starch and skimmed milk powder for further metals determination by spectrometric plasma-based techniques. The sample digestion was conducted using an in situ UV lamp (electrodeless discharge lamp), and the digestion efficiency was improved by employing O2 (20 bar) and 2 mL 30 % H2O2 as auxiliary reagents. The accuracy of the proposed digestion method was evaluated by metals determination (Ca, Cd, Cu, Fe, K, Mg, Mo, Mn, Na, Pb, and Zn) in certificated reference material, which agreed with certified values (Student t-test <0,05). With the use of a UV lamp an environmentally friendly protocol was developed for starch and skimmed milk powder digestion using 0.1 mol L-1 HNO3 with auxiliary reagents (H2O2 or O2). The RCC value ranged from 0.9 to 1.2 % (starch and skimmed milk powder, respectively). The simultaneous cooling approach further improved the digestion efficiency (RCC <0,3 % for both samples), allowing to use milder digestion conditions, or even just water, being environmentally friendly, reducing the waste generation and reagents consumption, allowing food quality control through a greener approach.

A Comparative Analysis of Sparisoma cretense in Island Environments: Unraveling Metal Accumulation Differences in the Canary Islands (Spain, NW African Waters).

This study investigates the impact of varying environmental conditions on the metal composition within the tissues of Sparisoma cretense, contributing to the understanding necessary to offer scientifically sound advice regarding the health status of this species. This knowledge extends beyond fishery production, encompassing implications for food security. The data span the years 2022 and 2023, encompassing both cold and warm climatic seasons. The concentrations of various metals, such as Al, Zn, Cd, Pb, Fe, and Cu, exhibited noteworthy variations across the islands, with significant increases recorded in 2023, particularly during the warm season. The intricate interplay between multiple factors shaped the availability of the analyzed elements in S. cretense. Factors such as rising temperatures during the warm season increased biological activity in marine ecosystems, seasonal fluctuations in weather conditions, water quality, and anthropogenic influences, all contributing to the observed variations in metal concentrations. Additionally, the geological composition of each island and the patterns of marine currents and sediment transport play pivotal roles in these differences. Comprehensive scientific research, monitoring, and environmental surveillance are essential for a holistic understanding of this variability and providing valuable insights for the conservation and management of marine ecosystems in the Canary archipelago.

Spectrochemical Analysis of Nutritional and Toxic Metals in Different Brands of Candies Using Advanced Diagnostic Approaches.

Candies are frequently consumed sweetened food products among children, and their consumption can expose them to potential toxins and contamination. The present study employed calibration-free-laser-induced breakdown spectroscopy (CF-LIBS) as a steadfast diagnostic technique to analyze the presence of nutritional and heavy metals in candies from various brands available in local markets in Haripur, Pakistan. A pulsed Nd-YAG laser was used to create a microplasma on the target surface. The emitted light was collected using a dedicated spectrometer to analyze and quantify the species present in the candies. The analysis revealed the presence of micronutrients, macronutrients, and trace toxic metals such as Ca, K, Mg, Fe, Na, Al, Pb, Cr, Mn, Zn, Cu, and Ni. The optimization of our LIBS system was achieved through a parametric dependence study. The confirmation of the assumption of local thermodynamic equilibrium was achieved by assessing McWhirter's criterion and considering the relaxation time and the diffusion length of atoms in plasma. The highest concentrations (measured in mg/kg) of Pb, Cr, and Mn were determined to be 15 mg/kg, 340 mg/kg, and 880 mg/kg, respectively. The levels of Pb and Cr were found to be well above the maximum accepted limit set by the WHO. Similarly, the concentration of Mn in S-4 and S-5 exceeded the permissible limits set by the WHO. However, Zn, Cu, and Ni were found within safe limits in all the collected samples. In addition, the abundance obtained through CF-LIBS was compared to the concentration of similar (duplicate) candies samples analyzed using a standard analytical technique like inductively coupled plasma-optical emission spectroscopy. Excellent harmony could be seen in both outcomes. Moreover, energy-dispersive X-ray spectroscopy (EDX) was also utilized to confirm the presence of detected toxins. The significance of our findings lies in creating awareness among the public about the health risks associated with consuming toxins through candies, thereby protecting numerous human lives.